Microbial Culture Facility and Repository

Our microbial culture facility and Repository has the following equipment available (housed in either MR-4 or MR-6). For access to any of this equipment, please reach out the Casandra Hoffman (clh4he@virginia.edu).

 

  • Bio-Rad CFX96 real-time PCR detection systems, Eppendorf conventional gradient PCR instruments, OptiMax thermocycler (MR-4)
  • Class II Biosafety cabinets for handling of bacterial pathogens (MR-4)
  • Incubators, shaking incubators, Fisher Scientific jacketed CO2 incubators (MR-4)
  • Refrigerators and -20°C freezers (MR-4)
  • Eppendorf microcentrifuges, Beckman L7-55 ultracentrifuge, Sorvall refrigerated benchtop centrifuge, Avanti J-30I centrifuge with multiple rotors, International PR-J swinging bucket centrifuge, Sorvall RC5B Superspeed centrifuge (MR-4)
  • Milli-Q water purification system (MR-4)
  • Vortex mixers, pH meter, hot plate stirrers, balances including Mettler balance (MR-4)
  • Ultrasonic bath sonicator and Ultrasonic W-375 sonicator/cell disruptor (MR-4)
  • Fisher Isotemp water bath (MR-4)
  • Autoclave and dishwashing facility (MR-4)
  • Spectronic 601 spectrophotometer (MR-4)
  • Bio-Rad Gel Doc system (MR-4)
  • Quibit Flex Fluorometer (MR-4)
  • Biospec Mini-Beadbeater-96 (MR-4)
  • Qiagen TissueLyser II (MR-6)
  • Illumina MiSeq (MR-4)
  • Tecan Infinite M Plex Plate Reader (MR-4)
  • 8 Cerillo Stratus Plate Readers (MR-4)
  • 3 Coy Anaerobic Chambers, two Type-B double chambers (MR-4 and MR-6), one Type-A single chamber (Fontaine Research Park, Gnotobiotic Animal Facility)
  • Opentrons OT-2 Liquid Handling Robot (MR-4)
  • Opetrons Next Generation Sequencing Robot (MR-4)
  • 4 165 Liter Panasonic temperature- and CO2- controlled incubators (MR-4)
  • 4 1300 Series A2 Biosafety Cabinets (MR-4)
  • BioTek ELx50 ELISA Plate Washer (MR-4)
  • SYNERGY HTX multi-mode (fluorescence, luminescence) plate reader (MR-4)
  • a Sartorius weigh scale, two Denver Instrument weigh scales (MR-4)
  • upright Fisher microscope with digital camera (MR-4)
  • Olympus BX41 light microscope, a dissecting microscope (MR-4)
  • PTC-100 Programmable Thermal Controller PCR Machine (MR-4)
  • BIO-RAD iCyclerIQ multicolor Real-Time PCR Detection System (MR-4)
  • Benchmark MyBlock Eppendorf tube heat block (MR-4)
  • Eppendorf Thermomixer heat block/sample mixer (MR-4)
  • Perkin Elmer UV/Vis Spectrophotometer Lambda Bio (MR-4)
  • 2 Kronos Dio AB-2550 luminometers with CO2 and temperature control (MR-4)
  • Fisher Scientific accuSpin Micro 17 benchtop centrifuge (MR-4)
  • Glite UV gel imager (MR-4)
  • Eppendorf 5810 R Table-Top Centrifuge (MR-4)
  • Fisher Scientific AR15 pH meter (MR-4)
  • four hot plate stirrers (MR-4)
  • two water baths (MR-4)
  • MVE Cryosystem 750 liquid nitrogen storage tank, and a  Thermo Fisher Scientific CY509108 4000 vial liquid nitrogen storage tank (MR-4)
  • Eppendorf CryoCube Ultra-Low Temperature -80ºC freezer in a common equipment area (MR-4)
  • Evos Microscope/Cell Imaging System with 5 color cubes and up to 100X magnification (MR-4)
  • 60 square foot, walk-in cold room (MR-4)

 

Coming Soon Sequencing Services
Coming Soon Sequencing Services